Title | The Unmixing Problem: A Guide to Applying Single-Cell RNA Sequencing to Bone. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Greenblatt MB, Ono N, Ayturk UM, Debnath S, Lalani S |
Journal | J Bone Miner Res |
Volume | 34 |
Issue | 7 |
Pagination | 1207-1219 |
Date Published | 2019 07 |
ISSN | 1523-4681 |
Keywords | Animals, Bone and Bones, Humans, Molecular Sequence Annotation, Reproducibility of Results, Sequence Analysis, RNA, Single-Cell Analysis |
Abstract | Bone is composed of a complex mixture of many dynamic cell types. Flow cytometry and in vivo lineage tracing have offered early progress toward deconvoluting this heterogeneous mixture of cells into functionally well-defined populations suitable for further studies. Single-cell sequencing is poised as a key complementary technique to better understand the cellular basis of bone metabolism and development. However, single-cell sequencing approaches still have important limitations, including transcriptional effects of cell isolation and sparse sampling of the transcriptome, that must be considered during experimental design and analysis to harness the power of this approach. Accounting for these limitations requires a deep knowledge of the tissue under study. Therefore, with the emergence of accessible tools for conducting and analyzing single-cell RNA sequencing (scRNA-seq) experiments, bone biologists will be ideal leaders in the application of scRNA-seq to the skeleton. Here we provide an overview of the steps involved with a single-cell sequencing analysis of bone, focusing on practical considerations needed for a successful study. © 2019 American Society for Bone and Mineral Research. |
DOI | 10.1002/jbmr.3802 |
Alternate Journal | J Bone Miner Res |
PubMed ID | 31336008 |
PubMed Central ID | PMC6658136 |
Grant List | R01DE026666 / NH / NIH HHS / United States Career Award for Medical Scientists / / Burroughs Wellcome Fund / International DP5OD021351 / NH / NIH HHS / United States R01 DE026666 / DE / NIDCR NIH HHS / United States Pershing Square Sohn Cancer Research Alliance / / Pershing Square Sohn Prize for Young Investigators in Cancer Research / International DP5 OD021351 / OD / NIH HHS / United States R01 AR075585 / AR / NIAMS NIH HHS / United States Basil O'Connor Award / / March of Dimes Foundation / International |
Related Faculty:
Matthew B. Greenblatt, M.D., Ph.D.