| Title | S1P/S1P1 signaling stimulates cell migration and invasion in Wilms tumor. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Li M-H, Sanchez T, Yamase H, Hla T, Oo MLin, Pappalardo A, Lynch KR, Lin C-Y, Ferrer F |
| Journal | Cancer Lett |
| Volume | 276 |
| Issue | 2 |
| Pagination | 171-9 |
| Date Published | 2009 Apr 18 |
| ISSN | 1872-7980 |
| Keywords | Cell Line, Tumor, Cell Movement, GTP-Binding Protein alpha Subunits, Gi-Go, Humans, Kidney Neoplasms, Lysophospholipids, Neoplasm Invasiveness, Phosphatidylinositol 3-Kinases, rac1 GTP-Binding Protein, Receptors, Lysosphingolipid, RNA, Small Interfering, Signal Transduction, Sphingosine, Wilms Tumor |
| Abstract | Sphingosine-1-phosphate (S1P) is an important regulator of cellular functions via interaction with its receptors S1P(1-5). To date, nothing is known about the S1P receptor expression and the effects of S1P signaling in Wilms tumor. In this study, we found ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. We demonstrated that S1P(1) acted as a promigratory modulator by employing S1P(1) antagonist VPC44116, S1P(1) siRNA and adenoviral transduction in Wilms tumor cells. Further, we clarified that S1P(1)-mediated migration occurred via Gi coupling and activation of PI3K and Rac1. In addition, S1P stimulated WiT49 cell invasion through S1P(1)/Gi signaling pathway. We consider that targeting S1P(1) may be a point of therapeutic intervention in Wilms tumor. |
| DOI | 10.1016/j.canlet.2008.11.025 |
| Alternate Journal | Cancer Lett |
| PubMed ID | 19131156 |
| PubMed Central ID | PMC2943759 |
| Grant List | K08 DK070468-03 / DK / NIDDK NIH HHS / United States K08 DK 070468 A / DK / NIDDK NIH HHS / United States K08 DK070468 / DK / NIDDK NIH HHS / United States R01 CA168903 / CA / NCI NIH HHS / United States R01 GM067958-06 / GM / NIGMS NIH HHS / United States R01 GM067958 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Teresa Sanchez, Ph.D.