| Title | Phospholipase C-mediated hydrolysis of phosphatidylcholine is an important step in PDGF-stimulated DNA synthesis. |
| Publication Type | Journal Article |
| Year of Publication | 1990 |
| Authors | Larrodera P, Cornet ME, Diaz-Meco MT, Lopez-Barahona M, Diaz-Laviada I, Guddal PH, Johansen T, Moscat J |
| Journal | Cell |
| Volume | 61 |
| Issue | 6 |
| Pagination | 1113-20 |
| Date Published | 1990 Jun 15 |
| ISSN | 0092-8674 |
| Keywords | Animals, Bacillus cereus, Cells, Cultured, DNA, DNA Replication, Drug Synergism, Hydrolysis, Insulin, Kinetics, Mice, Phosphatidylcholines, Platelet-Derived Growth Factor, Tetradecanoylphorbol Acetate, Thymidine, Type C Phospholipases |
| Abstract | Recent evidence suggests the involvement of phosphatidylcholine (PC) hydrolysis both in the control of normal cell growth and in transformation. We show here that the simple exogenous addition of Bacillus cereus PC-hydrolyzing phospholipase C (PC-PLC) is sufficient to elicit a potent mitogenic response in Swiss 3T3 fibroblasts by a mechanism that is independent of protein kinase C. Our results on the additivity and synergism between B. cereus PC-PLC, PDGF, and insulin in the mitogenic response indicate that this novel phospholipid degradative pathway may be important in the mitogenic signaling cascade activated by PDGF. |
| DOI | 10.1016/0092-8674(90)90074-o |
| Alternate Journal | Cell |
| PubMed ID | 2112426 |
Related Faculty:
Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.