Title | Evaluation of whole genome amplification protocols for array and oligonucleotide CGH. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Hittelman A, Sridharan S, Roy R, Fridlyand J, Loda M, Collins C, Paris PL |
Journal | Diagn Mol Pathol |
Volume | 16 |
Issue | 4 |
Pagination | 198-206 |
Date Published | 2007 Dec |
ISSN | 1052-9551 |
Keywords | Female, Genome, Human, Humans, Male, Nucleic Acid Amplification Techniques, Oligonucleotide Array Sequence Analysis, Oligonucleotides, Sensitivity and Specificity |
Abstract | Genome-based technologies such as genomic arrays and next generation sequencing are poised to make significant contributions to clinical oncology. However, translation of these technologies to the clinic will require that they produce high-quality reproducible data from small archived tumor specimens and biopsies. Herein, we report on a systematic and comprehensive microarray analysis comparing multiple whole genome amplification methods using a variety of difficult clinical specimens, including formalin-fixed and paraffin-embedded tissues. Quantitative analysis and clustering suggest that Sigma's whole genome amplification protocol performed best on all specimens and, moreover, worked well with a formalin-fixed, paraffin-embedded biopsy. |
DOI | 10.1097/PDM.0b013e3180f62ad5 |
Alternate Journal | Diagn Mol Pathol |
PubMed ID | 18043282 |
Related Faculty:
Massimo Loda, M.D.