| Title | Determining protein half-lives. |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Zhou P |
| Journal | Methods Mol Biol |
| Volume | 284 |
| Pagination | 67-77 |
| Date Published | 2004 |
| ISSN | 1064-3745 |
| Keywords | Cycloheximide, Cysteine Endopeptidases, Fungal Proteins, Half-Life, HeLa Cells, Humans, Immunoblotting, Isotope Labeling, Methods, Multienzyme Complexes, Promoter Regions, Genetic, Proteasome Endopeptidase Complex, Protein Processing, Post-Translational, Protein Synthesis Inhibitors, Proteins, Yeasts |
| Abstract | Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein's half-life in yeast and cultured mammalian cells. |
| DOI | 10.1385/1-59259-816-1:067 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 15173609 |
Related Faculty:
Pengbo Zhou, Ph.D.