Title | Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Jima DD, Zhang J, Jacobs C, Richards KL, Dunphy CH, Choi WWL, Au WYan, Srivastava G, Czader MB, Rizzieri DA, Lagoo AS, Lugar PL, Mann KP, Flowers CR, Bernal-Mizrachi L, Naresh KN, Evens AM, Gordon LI, Luftig M, Friedman DR, J Weinberg B, Thompson MA, Gill JI, Liu Q, How T, Grubor V, Gao Y, Patel A, Wu H, Zhu J, Blobe GC, Lipsky PE, Chadburn A, Dave SS |
Corporate Authors | Hematologic Malignancies Research Consortium |
Journal | Blood |
Volume | 116 |
Issue | 23 |
Pagination | e118-27 |
Date Published | 2010 Dec 02 |
ISSN | 1528-0020 |
Keywords | B-Lymphocytes, Base Sequence, Chromatin Immunoprecipitation, Gene Expression Profiling, Gene Library, High-Throughput Nucleotide Sequencing, Humans, Lymphoma, Large B-Cell, Diffuse, MicroRNAs, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, RNA |
Abstract | A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing of 31 normal and malignant human B-cell samples that comprise the spectrum of B-cell differentiation and common malignant phenotypes. We identified the expression of 333 known miRNAs, which is more than twice the number previously recognized in any tissue type. We further identified the expression of 286 candidate novel miRNAs in normal and malignant B cells. These miRNAs were validated at a high rate (92%) using quantitative polymerase chain reaction, and we demonstrated their application in the distinction of clinically relevant subgroups of lymphoma. We further demonstrated that a novel miRNA cluster, previously annotated as a hypothetical gene LOC100130622, contains 6 novel miRNAs that regulate the transforming growth factor-β pathway. Thus, our work suggests that more than a third of the miRNAs present in most cellular types are currently unknown and that these miRNAs may regulate important cellular functions. |
DOI | 10.1182/blood-2010-05-285403 |
Alternate Journal | Blood |
PubMed ID | 20733160 |
Related Faculty:
Amy Chadburn, M.D.