Title | Combined heparin/acid citrate dextrose solution A anticoagulation in the Optia continuous mononuclear cell protocol for pediatric lymphocyte apheresis. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | DeSimone RA, Myers GD, Guest EM, Shi PA |
Journal | J Clin Apher |
Volume | 34 |
Issue | 4 |
Pagination | 487-489 |
Date Published | 2019 Aug |
ISSN | 1098-1101 |
Keywords | Anticoagulants, Child, Citric Acid, Glucose, Heparin, Humans, Immunotherapy, Adoptive, Infant, Leukapheresis, Leukocytes, Mononuclear, Pediatrics, T-Lymphocytes |
Abstract | Collecting a sufficient number of T-cells is a critical first step in the production of chimeric antigen receptor (CAR)-T cells. Herein, we report a successful implementation of anticoagulation with combined heparin and acid citrate dextrose solution A (ACD-A) for the continuous mononuclear cell (CMNC) protocol on the Spectra Optia in a 20-month-old, 7.5 kg patient with refractory acute lymphoblastic leukemia for manufacture of tisagenlecleucel, a CAR-T cell therapy. Combined heparin/ACD-A was used following clotting issues when ACD-A was used alone during initial CMNC collections. To our knowledge, this is the first reported case in pediatrics of combined heparin/ACD-A anticoagulation with the Spectra Optia CMNC protocol. |
DOI | 10.1002/jca.21675 |
Alternate Journal | J Clin Apher |
PubMed ID | 30489654 |
Related Faculty:
Robert DeSimone, M.D.