Title | CMV antigen-specific CD4+ and CD8+ T cell IFNgamma expression and proliferation responses in healthy CMV-seropositive individuals. |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | Sinclair E, Black D, C Epling L, Carvidi A, Josefowicz SZ, Bredt BM, Jacobson MA |
Journal | Viral Immunol |
Volume | 17 |
Issue | 3 |
Pagination | 445-54 |
Date Published | 2004 |
ISSN | 0882-8245 |
Keywords | Antibodies, Viral, Antigens, Viral, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cytomegalovirus, Cytomegalovirus Infections, Humans, Interferon-gamma, Lymphocyte Activation, Sensitivity and Specificity |
Abstract | CMV-specific CD4+ and CD8+ T cell IFNgamma expression and proliferation were measured in healthy volunteers by flow cytometry after CMV lysate or CMV pp65 or IE peptide pool stimulation. Cutoff values were set to maximize specificity (i.e., no false positive CMV-seronegatives). Sensitivity (defined as a positive response in CMV-seropositives to at least one of the 3 antigen preparations used) was 100% for CMV-specific CD4+ and CD8+ T cell IFN expression and CD4+ T cell proliferation and 95.4% for CMV-specific CD8+ T cell proliferation. All 22 CMV-seropositive individuals had positive responses by at least three of these four measurements. These findings support the concept that a multiplicity of antigen-specific functional immune responses and persistence of robust virus-specific CD4+T cells are important components of protective immunity in this chronic viral infection. |
DOI | 10.1089/vim.2004.17.445 |
Alternate Journal | Viral Immunol |
PubMed ID | 15357911 |
PubMed Central ID | PMC1993826 |
Grant List | R01 AI049538-01A1 / AI / NIAID NIH HHS / United States R01 AI049538 / AI / NIAID NIH HHS / United States R01 AI049538-03 / AI / NIAID NIH HHS / United States R01AI47062 / AI / NIAID NIH HHS / United States R0-1 AI49538 / AI / NIAID NIH HHS / United States M01 RR00083 / RR / NCRR NIH HHS / United States P30 AI027763 / AI / NIAID NIH HHS / United States M01 RR000083 / RR / NCRR NIH HHS / United States R01 AI047062 / AI / NIAID NIH HHS / United States P30 AI27763 / AI / NIAID NIH HHS / United States R01 AI049538-02 / AI / NIAID NIH HHS / United States |
Related Faculty:
Steven Josefowicz, Ph.D.