Title | A multisite performance study comparing the reading of immunohistochemical slides on a computer monitor with conventional manual microscopy for estrogen and progesterone receptor analysis. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Nassar A, Cohen C, Agersborg SS, Zhou W, Lynch KA, Barker EA, Vanderbilt BL, Thompson J, Heyman ER, Olson A, Lange H, Siddiqui MT |
Journal | Am J Clin Pathol |
Volume | 135 |
Issue | 3 |
Pagination | 461-7 |
Date Published | 2011 Mar |
ISSN | 1943-7722 |
Keywords | Biomarkers, Tumor, Breast Neoplasms, Cell Nucleus, Female, Humans, Image Processing, Computer-Assisted, Immunohistochemistry, Microscopy, Observer Variation, Receptors, Estrogen, Receptors, Progesterone, Reproducibility of Results, Telepathology |
Abstract | A multisite study was conducted to assess the performance of the Aperio digital pathology system (Aperio Technologies, Vista, CA) for reading estrogen receptor (ER) and progesterone receptor (PR) slides on a computer monitor. A total of 520 formalin-fixed breast tissue specimens were assayed at 3 clinical sites for ER and PR (260 each). Percentage and average staining intensity of positive nuclei were assessed. At each site, 3 pathologists performed a blinded reading of the glass slides using their microscopes initially and later using digital images on a computer monitor. Comparable percentages of agreements were obtained for manual microscopy (MM) and manual digital slide reading (MDR) (ER, percentage of positive nuclei with cutoffs: MM, 91.3%-99.0%/MDR, 91.3%-100.0%; PR, percentage of positive nuclei with cutoffs: MM, 83.8%-99.0%/MDR, 76.3%-100.0%). Reading ER and PR slides on a computer monitor using the Aperio digital pathology system is equivalent to reading the slides with a conventional light microscope. |
DOI | 10.1309/AJCP4VFKA5FCMZNA |
Alternate Journal | Am J Clin Pathol |
PubMed ID | 21350103 |
Related Faculty:
Momin Siddiqui, M.D.