| Title | Wnt5 signaling in vertebrate pancreas development. |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | Kim HJ, Schleiffarth JR, Jessurun J, Sumanas S, Petryk A, Lin S, Ekker SC |
| Journal | BMC Biol |
| Volume | 3 |
| Pagination | 23 |
| Date Published | 2005 Oct 24 |
| ISSN | 1741-7007 |
| Keywords | Animals, Base Sequence, Cell Movement, Embryo, Nonmammalian, Frizzled Receptors, Gene Expression Regulation, Developmental, In Situ Hybridization, Insulin, Islets of Langerhans, Mice, Mice, Knockout, Morphogenesis, Pancreas, Polymerase Chain Reaction, Proto-Oncogene Proteins, Receptors, G-Protein-Coupled, Recombinant Fusion Proteins, Signal Transduction, Transcription, Genetic, Transfection, Vertebrates, Wnt Proteins, Wnt-5a Protein, Xenopus, Zebrafish, Zebrafish Proteins |
| Abstract | BACKGROUND: Signaling by the Wnt family of secreted glycoproteins through their receptors, the frizzled (Fz) family of seven-pass transmembrane proteins, is critical for numerous cell fate and tissue polarity decisions during development. RESULTS: We report a novel role of Wnt signaling in organogenesis using the formation of the islet during pancreatic development as a model tissue. We used the advantages of the zebrafish to visualize and document this process in living embryos and demonstrated that insulin-positive cells actively migrate to form an islet. We used morpholinos (MOs), sequence-specific translational inhibitors, and time-lapse imaging analysis to show that the Wnt-5 ligand and the Fz-2 receptor are required for proper insulin-cell migration in zebrafish. Histological analyses of islets in Wnt5a(-/-) mouse embryos showed that Wnt5a signaling is also critical for murine pancreatic insulin-cell migration. CONCLUSION: Our results implicate a conserved role of a Wnt5/Fz2 signaling pathway in islet formation during pancreatic development. This study opens the door for further investigation into a role of Wnt signaling in vertebrate organ development and disease. |
| DOI | 10.1186/1741-7007-3-23 |
| Alternate Journal | BMC Biol |
| PubMed ID | 16246260 |
| PubMed Central ID | PMC1276788 |
| Grant List | GM63904 / GM / NIGMS NIH HHS / United States R01 GM063904-01 / GM / NIGMS NIH HHS / United States R01 GM055877 / GM / NIGMS NIH HHS / United States R01 GM063904 / GM / NIGMS NIH HHS / United States R56 GM063904 / GM / NIGMS NIH HHS / United States T32 GM008244 / GM / NIGMS NIH HHS / United States 5 T32 GM08244-16 / GM / NIGMS NIH HHS / United States GM55877 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Jose Jessurun, M.D.