Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer.

TitleWhole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer.
Publication TypeJournal Article
Year of Publication2014
AuthorsLohr JG, Adalsteinsson VA, Cibulskis K, Choudhury AD, Rosenberg M, Cruz-Gordillo P, Francis JM, Zhang C-Z, Shalek AK, Satija R, Trombetta JJ, Lu D, Tallapragada N, Tahirova N, Kim S, Blumenstiel B, Sougnez C, Lowe A, Wong B, Auclair D, Van Allen EM, Nakabayashi M, Lis RT, Lee G-SM, Li T, Chabot MS, Ly A, Taplin M-E, Clancy TE, Loda M, Regev A, Meyerson M, Hahn WC, Kantoff PW, Golub TR, Getz G, Boehm JS, J Love C
JournalNat Biotechnol
Volume32
Issue5
Pagination479-84
Date Published2014 May
ISSN1546-1696
KeywordsExome, Humans, Male, Mutation, Neoplastic Cells, Circulating, Prostatic Neoplasms
Abstract

Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. A major barrier, however, is inaccessibility of metastatic tissue. A potential solution is to characterize circulating tumor cells (CTCs), but this requires overcoming the challenges of isolating rare cells and sequencing low-input material. Here we report an integrated process to isolate, qualify and sequence whole exomes of CTCs with high fidelity using a census-based sequencing strategy. Power calculations suggest that mapping of >99.995% of the standard exome is possible in CTCs. We validated our process in two patients with prostate cancer, including one for whom we sequenced CTCs, a lymph node metastasis and nine cores of the primary tumor. Fifty-one of 73 CTC mutations (70%) were present in matched tissue. Moreover, we identified 10 early trunk and 56 metastatic trunk mutations in the non-CTC tumor samples and found 90% and 73% of these mutations, respectively, in CTC exomes. This study establishes a foundation for CTC genomics in the clinic.

DOI10.1038/nbt.2892
Alternate JournalNat Biotechnol
PubMed ID24752078
Grant List5P50CA100707-10 / CA / NCI NIH HHS / United States
P30-CA14051 / CA / NCI NIH HHS / United States
Related Faculty: 
Massimo Loda, M.D.

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