In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences.

TitleIn vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences.
Publication TypeJournal Article
Year of Publication1995
AuthorsCesarman E, Moore PS, Rao PH, Inghirami G, Knowles DM, Chang Y
JournalBlood
Volume86
Issue7
Pagination2708-14
Date Published1995 Oct 01
ISSN0006-4971
KeywordsBase Sequence, Blotting, Southern, Cell Nucleus, Cytoplasm, DNA Restriction Enzymes, DNA, Viral, Genotype, Humans, Immunophenotyping, In Situ Hybridization, Lymphoma, AIDS-Related, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, IgE, Sarcoma, Kaposi, Sequence Homology, Simplexvirus, Tumor Cells, Cultured
Abstract

Two unique DNA fragments were recently identified in over 90% of acquired immunodeficiency syndrome (AIDS)-related Kaposi's sarcoma tissues. Sequence analysis suggests that these fragments belong to a previously unidentified human herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV). These fragments have also been identified in a subset of non-Hodgkin's lymphomas in human immunodeficiency virus-positive patients; specifically, in body cavity-based lymphomas (AIDS-BCBLs). We have established two cell lines derived from AIDS-BCBLs, BC-1 and BC-2, which retain the KSHV sequences, and have used them to further characterize this putative viral genome. In this report, we demonstrate that the KSHV sequences represent a portion of a much larger DNA molecule that is located predominantly in the nucleus of the infected cells. In situ hybridization of metaphase spreads made from both of these cell lines show these sequences in episomal structures. Their presence in the cells as large nuclear episomes supports previous sequence homology data suggesting that KSHV belongs to the herpesvirus family. These cell lines provide a continuous source of KSHV sequences. Thus, they represent an important tool for future studies of this recently described human herpesvirus that may be involved in the pathogenesis of Kaposi's sarcoma and some AIDS-related non-Hodgkin's lymphomas.

Alternate JournalBlood
PubMed ID7670109
Grant ListEY06337 / EY / NEI NIH HHS / United States
Related Lab: 
Related Faculty: 
Ethel Cesarman, M.D., Ph.D. Giorgio Inghirami, M.D.

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