| Title | Two-photon 3D FIONA of individual quantum dots in an aqueous environment. |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Zhang R, Rothenberg E, Fruhwirth G, Simonson PD, Ye F, Golding I, Ng T, Lopes W, Selvin PR |
| Journal | Nano Lett |
| Volume | 11 |
| Issue | 10 |
| Pagination | 4074-8 |
| Date Published | 2011 Oct 12 |
| ISSN | 1530-6992 |
| Keywords | Breast Neoplasms, Endocytosis, ErbB Receptors, Female, Humans, Photons, Quantum Dots, Water |
| Abstract | We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9 × 9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells. |
| DOI | 10.1021/nl201225r |
| Alternate Journal | Nano Lett |
| PubMed ID | 21882883 |
| Grant List | R01GM082837 / GM / NIGMS NIH HHS / United States GM086214 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Paul Simonson, M.D., Ph.D.