Title | A thermo-sensitive fluorescent agent based method for excitation light leakage rejection for fluorescence molecular tomography. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Nouizi F, Kwong TC, Ruiz J, Cho J, Chan Y-W, Ikemura K, Erkol H, Sampathkumaran U, Gulsen G |
Journal | Phys Med Biol |
Volume | 64 |
Issue | 3 |
Pagination | 035007 |
Date Published | 2019 Jan 22 |
ISSN | 1361-6560 |
Keywords | Algorithms, Fluorescent Dyes, Light, Phantoms, Imaging, Temperature, Tomography |
Abstract | Fluorescence molecular tomography (FMT) is widely used in preclinical oncology research. FMT is the only imaging technique able to provide 3D distribution of fluorescent probes within thick highly scattering media. However, its integration into clinical medicine has been hampered by its low spatial resolution caused by the undetermined and ill-posed nature of its reconstruction algorithm. Another major factor degrading the quality of FMT images is the large backscattered excitation light component leaking through the rejection filters and coinciding with the weak fluorescent signal arising from a low tissue fluorescence concentration. In this paper, we present a new method based on the use of a novel thermo-sensitive fluorescence probe. In fact, the excitation light leakage is accurately estimated from a set of measurements performed at different temperatures and then is corrected for in the tomographic data. The obtained results show a considerable improvement in both spatial resolution and quantitative accuracy of FMT images due to the proper correction of fluorescent signals. |
DOI | 10.1088/1361-6560/aaf96d |
Alternate Journal | Phys Med Biol |
PubMed ID | 30561380 |
PubMed Central ID | PMC7781084 |
Grant List | R21 CA170955 / CA / NCI NIH HHS / United States R33 CA120175 / CA / NCI NIH HHS / United States F31 CA171915 / CA / NCI NIH HHS / United States R01 EB008716 / EB / NIBIB NIH HHS / United States P30 CA062203 / CA / NCI NIH HHS / United States |
Related Faculty:
Kenji Ikemura, M.D.