Title | Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Yang HSarina, Edinboro L |
Journal | Methods Mol Biol |
Volume | 1872 |
Pagination | 67-73 |
Date Published | 2019 |
ISSN | 1940-6029 |
Keywords | Anticonvulsants, Chromatography, Liquid, Data Interpretation, Statistical, Drug Monitoring, Humans, Lacosamide, Tandem Mass Spectrometry |
Abstract | High-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) has become a primary analytical methodology in therapeutic drug monitoring of antiepileptic drugs (AEDs). To demonstrate the utility of LC-MS/MS in measuring drug concentrations in serum or plasma, analysis of lacosamide (Vimpat) is discussed in this chapter. Lacosamide is an example of the newer-generation AEDs. The drug is extracted by protein precipitation and dilution of the serum specimen. A small volume of the extracted specimen is injected into a reversed-phase chromatography column, and lacosamide is identified by positive electrospray ionization (ESI) mass spectrometry in the multiple reaction monitoring (MRM) mode, which provides selectivity for quantitative analysis. A deuterated internal standard is used to correct for any loss of analyte during the process of extraction and analysis. A seven-point calibration curve and two levels of quality controls are included in each batch. |
DOI | 10.1007/978-1-4939-8823-5_7 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 30350280 |
Related Faculty:
He Sarina Yang, M.D., Ph.D.