| Title | Studies of the Escherichia coli Rsd-sigma70 complex. |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Westblade LF, Ilag LL, Powell AK, Kolb A, Robinson CV, Busby SJW |
| Journal | J Mol Biol |
| Volume | 335 |
| Issue | 3 |
| Pagination | 685-92 |
| Date Published | 2004 Jan 16 |
| ISSN | 0022-2836 |
| Keywords | Binding Sites, DNA-Directed RNA Polymerases, Electrophoresis, Polyacrylamide Gel, Escherichia coli Proteins, Molecular Weight, Mutagenesis, Site-Directed, Protein Binding, Repressor Proteins, Sigma Factor, Spectrometry, Mass, Electrospray Ionization |
| Abstract | Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex. |
| DOI | 10.1016/j.jmb.2003.11.004 |
| Alternate Journal | J Mol Biol |
| PubMed ID | 14687566 |
Related Faculty:
Lars Westblade, Ph.D.