Title | Polymerase chain reaction-based methods for the detection of mutations in oncogenes and tumor suppressor genes. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Loda M |
Journal | Hum Pathol |
Volume | 25 |
Issue | 6 |
Pagination | 564-71 |
Date Published | 1994 Jun |
ISSN | 0046-8177 |
Keywords | Base Sequence, Blotting, Southern, DNA Mutational Analysis, Genes, Tumor Suppressor, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Proto-Oncogenes |
Abstract | Altered expression of oncogenes and tumor suppressor genes, the normal function of which is to regulate cell growth and differentiation, represents a central event in the pathogenesis of human cancer. Aberrant expression of these genes is often a result of a mutational event. In vitro amplification of DNA with the polymerase chain reaction (PCR) has enormously increased the sensitivity of the methods to detect mutations. These PCR-based techniques have thus become invaluable in the elucidation of the mechanisms of carcinogenesis as well as in molecular genetics. In addition, the precise definition of a mutation at the molecular level can be a very valuable adjunct to the diagnosis and classification of malignancies as well as to their prognostic assessment. In this article several PCR-based strategies are outlined, their applicability in the detection of different types of mutations is discussed, and finally the application of these techniques in fresh and archival tissues is presented. |
DOI | 10.1016/0046-8177(94)90220-8 |
Alternate Journal | Hum Pathol |
PubMed ID | 7912220 |
Related Faculty:
Massimo Loda, M.D.