NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.

TitleNF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.
Publication TypeJournal Article
Year of Publication1992
AuthorsSpergel JM, Hsu W, Akira S, Thimmappaya B, Kishimoto T, Chen-Kiang S
JournalJ Virol
Date Published1992 Feb
KeywordsAdenovirus Early Proteins, Adenoviruses, Human, Base Sequence, Carcinoma, Hepatocellular, CCAAT-Enhancer-Binding Proteins, Cell Line, Chloramphenicol O-Acetyltransferase, DNA-Binding Proteins, Gene Expression Regulation, Viral, Humans, Interleukin-6, Liver Neoplasms, Molecular Sequence Data, Nuclear Proteins, Oligodeoxyribonucleotides, Oncogene Proteins, Viral, Plasmids, Promoter Regions, Genetic, Repetitive Sequences, Nucleic Acid, Signal Transduction, Trans-Activators, Transcription Factors, Transcriptional Activation, Transfection

A cDNA encoding NF-IL6, an interleukin-6 (IL-6)-regulated human nuclear factor of the C/EBP family, is demonstrated to complement the transactivation function of E1A. The endogenous NF-IL6 level varies according to cell type and correlates positively with an IL-6-regulated cellular E1A-substituting activity that was described recently (J.M. Spergel and S. Chen-Kiang, Proc. Natl. Acad. Sci. USA 88:6472-6476, 1991). When expressed by transfection in cells which contain low levels of NF-IL6 and are incapable of complementing the function of E1A proteins, NF-IL6 also transactivates the E1A-responsive E2ae and E1B promoters, to the same magnitude as E1A. Activation by NF-IL6 is concentration dependent and sequence specific: mutational studies of the E2ae promoter suggest that the promoter-proximal NF-IL6 recognition site functions as a dominant negative regulatory site whereas the promoter-distal NF-IL6 recognition site is positively regulated at low NF-IL6 concentrations and negatively regulated when the NF-IL6 level is high. Consistent with these functions, NF-IL6 alone is sufficient to complement an E1A deletion mutant dl312 in viral infection, when expressed at appropriate concentrations. These results identify NF-IL6 as a sequence-specific cellular nuclear factor which regulates E1A-responsive genes in the absence of E1A.

Alternate JournalJ Virol
PubMed ID1309887
PubMed Central IDPMC240805
Grant ListAI-24404 / AI / NIAID NIH HHS / United States
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Selina Chen-Kiang, Ph.D.

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