Title | Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage. |
Publication Type | Journal Article |
Year of Publication | 2023 |
Authors | Aguilar R, Khan L, Arslanovic N, Birmingham K, Kasliwal K, Posnikoff S, Chakraborty U, Hickman AR, Watson R, Ezell RJ, Willis HE, Cowles MW, Garner R, Shim A, Gutierrez I, Marunde MR, Keogh M-C, Tyler JK |
Journal | Res Sq |
Date Published | 2023 Jul 28 |
Abstract | Tardigrades are remarkable in their ability to survive extreme environments. The damage suppressor (Dsup) protein is thought responsible for their extreme resistance to reactive oxygen species (ROS) generated by irradiation. Here we show that expression of Ramazzottius varieornatus Dsup in Saccharomyces cerevisiae reduces oxidative DNA damage and extends the lifespan of budding yeast exposed to chronic oxidative genotoxicity. This protection from ROS requires either the Dsup HMGN-like domain or sequences C-terminal to same. Dsup associates with no apparent bias across the yeast genome, using multiple modes of nucleosome binding; the HMGN-like region interacts with both the H2A/H2B acidic patch and H3/H4 histone tails, while the C-terminal region binds DNA. These findings give precedent for engineering an organism by physically shielding its genome to promote survival and longevity in the face of oxidative damage. |
DOI | 10.21203/rs.3.rs-3182883/v1 |
Alternate Journal | Res Sq |
PubMed ID | 37546815 |
PubMed Central ID | PMC10402244 |
Grant List | R44 GM136172 / GM / NIGMS NIH HHS / United States R44 GM117683 / GM / NIGMS NIH HHS / United States R44 HG010640 / HG / NHGRI NIH HHS / United States R44 CA212733 / CA / NCI NIH HHS / United States R01 CA095641 / CA / NCI NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States R35 GM139816 / GM / NIGMS NIH HHS / United States R01 AG079883 / AG / NIA NIH HHS / United States |
Related Faculty:
Jessica K. Tyler, Ph.D.