Major histocompatibility complex-restricted recognition of autologous chronic lymphocytic leukemia by tumor-specific T cells.

TitleMajor histocompatibility complex-restricted recognition of autologous chronic lymphocytic leukemia by tumor-specific T cells.
Publication TypeJournal Article
Year of Publication1993
AuthorsSherman W, Liu Z, Inghirami G, Reed EF, Harris PE, Suciu-Foca NM
JournalImmunol Res
Volume12
Issue4
Pagination338-48
Date Published1993
ISSN0257-277X
KeywordsAmino Acid Sequence, Antibodies, Monoclonal, Antigens, Neoplasm, B-Lymphocytes, Base Sequence, Blotting, Southern, CD4-Positive T-Lymphocytes, Cell Line, Transformed, Cytotoxicity Tests, Immunologic, Genes, Immunoglobulin, HLA Antigens, Humans, Leukemia, Lymphocytic, Chronic, B-Cell, Lymphocyte Activation, Male, Molecular Sequence Data, T-Lymphocytes
Abstract

From the peripheral blood of a patient with chronic lymphocytic leukemia (CLL) we generated a T-cell line and clones which recognized autologous CLL. The line comprised T-cell clones which responded to the CLL as well as to autologous Epstein-Barr virus (EBV)-transformed B cells in an HLA-DR-restricted fashion. In addition, the line comprised clones which were CLL-specific and showed no reactivity against EBV-transformed B cells and against autologous peripheral blood mononuclear cells obtained during remission. The proliferative response of the CLL-specific T-cell clone was inhibited by monoclonal antibodies to HLA-DR11, the major histocompatibility complex (MHC)-restrictive element. These results indicate that the MHC class-II molecule of CLL binds a tumor-specific peptide which is recognized by autologous T cells in an MHC class-II-restricted fashion. Such a peptide may serve as a target for immunotherapy.

DOI10.1007/BF02935507
Alternate JournalImmunol Res
PubMed ID7908684
Grant ListR01-A125210-06 / / PHS HHS / United States
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