Title | Intracellular and extracellular expression of the major inducible 70kDa heat shock protein in experimental ischemia-reperfusion injury of the spinal cord. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Awad H, Suntres Z, Heijmans J, Smeak D, Bergdall-Costell V, Christofi FL, Magro C, Oglesbee M |
Journal | Exp Neurol |
Volume | 212 |
Issue | 2 |
Pagination | 275-84 |
Date Published | 2008 Aug |
ISSN | 1090-2430 |
Keywords | Animals, Disease Models, Animal, Dogs, Gene Expression, Gene Expression Regulation, Granulocyte Colony-Stimulating Factor, HSP70 Heat-Shock Proteins, Interleukin-3, Lipid Peroxides, Motor Neurons, Recombinant Fusion Proteins, Recombinant Proteins, Reperfusion Injury, Spinal Cord, Spinal Cord Ischemia, Thromboxane B2, Time Factors |
Abstract | Inflammatory responses exacerbate ischemia-reperfusion (IR) injury of spinal cord, although understanding of mediators is incomplete. The major inducible 70kDa heat shock protein (hsp70) is induced by ischemia and extracellular hsp70 (e-hsp70) can modulate inflammatory responses, but there is no published information regarding e-hsp70 levels in the cerebrospinal fluid (CSF) or serum as part of any neurological disease state save trauma. The present work addresses this deficiency by examining e-hsp70 in serum and CSF of dogs in an experimental model of spinal cord IR injury. IR injury of spinal cord caused hind limb paraplegia within 2-3 h that was correlated to lumbosacral poliomalacia with T cell infiltrates at 3 d post-ischemia. In this context, we showed a 5.2-fold elevation of e-hsp70 in CSF that was induced by ischemia and was sustained for the following 3 d observation interval. Plasma e-hsp70 levels were unaffected by IR injury, indicating e-hsp70 release from within the central nervous system. A putative source of this e-hsp70 was ependymal cells in the ischemic penumbra, based upon elevated i-hsp70 levels detected within these cells. Results warrant further investigation of e-hsp70's potential to modulate spinal cord IR injury. |
DOI | 10.1016/j.expneurol.2008.03.024 |
Alternate Journal | Exp Neurol |
PubMed ID | 18511046 |
Related Faculty:
Cynthia M. Magro, M.D.