| Title | Human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus is a new transmissible virus that infects B cells. |
| Publication Type | Journal Article |
| Year of Publication | 1996 |
| Authors | Mesri EA, Cesarman E, Arvanitakis L, Rafii S, Moore MA, Posnett DN, Knowles DM, Asch AS |
| Journal | J Exp Med |
| Volume | 183 |
| Issue | 5 |
| Pagination | 2385-90 |
| Date Published | 1996 May 01 |
| ISSN | 0022-1007 |
| Keywords | Acquired Immunodeficiency Syndrome, B-Lymphocytes, Blotting, Southern, Cell Line, DNA Probes, DNA, Viral, Fetal Blood, Genome, Viral, Herpesviridae, Herpesvirus 2, Saimiriine, Herpesvirus 4, Human, Humans, Polymerase Chain Reaction, Sarcoma, Kaposi |
| Abstract | Herpesviral DNA fragments isolated from AIDS-associated Kaposi's sarcoma (KS) tissue (KSHV-DNA) share homology with two lymphotropic oncogenic gamma-herpesviruses, Epstein-Barr virus and Herpesvirus saimiri, and are present in the lesions of more than 95% of HIV and non-HIV-associated forms of KS, AIDS-related body cavity-based lymphomas, and AIDS-related multicentric Castleman's disease. Here we show that BC-1, a KSHV-DNA-positive, body cavity-based lymphoma cell line, produces infective herpesviral particles carrying a linear 270-kb genome that specifically transmits KSHV-DNA to CD19+ B cells. Transmission of KSHV-DNA is dependent upon a biologically active, replicating virus, since it is blocked by UV irradiation and foscarnet, an inhibitor of viral DNA-polymerase. This study represents the first isolation and transmission of the human herpesvirus-8/KS-associated herpesvirus. |
| DOI | 10.1084/jem.183.5.2385 |
| Alternate Journal | J Exp Med |
| PubMed ID | 8642350 |
| PubMed Central ID | PMC2192551 |
| Grant List | AI-39192 / AI / NIAID NIH HHS / United States HL-02541 / HL / NHLBI NIH HHS / United States HL-44389 / HL / NHLBI NIH HHS / United States |
Related Faculty:
Ethel Cesarman, M.D., Ph.D.