Title | The histone chaperone Asf1p mediates global chromatin disassembly in vivo. |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | Adkins MW, Tyler JK |
Journal | J Biol Chem |
Volume | 279 |
Issue | 50 |
Pagination | 52069-74 |
Date Published | 2004 Dec 10 |
ISSN | 0021-9258 |
Keywords | Acetylation, Cell Cycle Proteins, Chromatin, Chromatin Assembly Factor-1, Chromosomal Proteins, Non-Histone, Deoxyribonuclease I, DNA, Fungal, DNA, Superhelical, DNA-Binding Proteins, Genes, Fungal, Histones, Micrococcal Nuclease, Molecular Chaperones, Mutation, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
Abstract | The packaging of the eukaryotic genome into chromatin is likely to be mediated by chromatin assembly factors, including histone chaperones. We investigated the function of the histone H3/H4 chaperones anti-silencing function 1 (Asf1p) and chromatin assembly factor 1 (CAF-1) in vivo. Analysis of chromatin structure by accessibility to micrococcal nuclease and DNase I digestion demonstrated that the chromatin from CAF-1 mutant yeast has increased accessibility to these enzymes. In agreement, the supercoiling of the endogenous 2mu plasmid is reduced in yeast lacking CAF-1. These results indicate that CAF-1 mutant yeast globally under-assemble their genome into chromatin, consistent with a role for CAF-1 in chromatin assembly in vivo. By contrast, asf1 mutants globally over-assemble their genome into chromatin, as suggested by decreased accessibility of their chromatin to micrococcal nuclease and DNase I digestion and increased supercoiling of the endogenous 2mu plasmid. Deletion of ASF1 causes a striking loss of acetylation on histone H3 lysine 9, but this is not responsible for the altered chromatin structure in asf1 mutants. These data indicate that Asf1p may have a global role in chromatin disassembly and an unexpected role in histone acetylation in vivo. |
DOI | 10.1074/jbc.M406113200 |
Alternate Journal | J Biol Chem |
PubMed ID | 15452122 |
Grant List | R01 GM064475 / GM / NIGMS NIH HHS / United States GM64475 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Jessica K. Tyler, Ph.D.