Expression of APOBEC family members as regulators of endogenous retroelements and malignant transformation in systemic autoimmunity.

TitleExpression of APOBEC family members as regulators of endogenous retroelements and malignant transformation in systemic autoimmunity.
Publication TypeJournal Article
Year of Publication2021
AuthorsMavragani CP, Kirou KA, Nezos A, Seshan S, Wild T, Wahl SM, Moutsopoulos HM, Crow MK
JournalClin Immunol
Volume223
Pagination108649
Date Published2021 02
ISSN1521-7035
KeywordsAutoimmunity, Cell Transformation, Neoplastic, Cells, Cultured, Cytidine Deaminase, Endogenous Retroviruses, Gene Expression Regulation, Humans, Interferons, Kidney, Leukocytes, Mononuclear, Lupus Erythematosus, Systemic, Lymphoma, Proteins, Salivary Glands, Sjogren's Syndrome
Abstract

OBJECTIVE: To explore whether APOBEC family members are involved in the response to inappropriate expression of L1 retroelements in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE), as well as in SS related lymphomagenesis.

METHODS: Minor salivary glands (MSG) and kidney biopsy (KB) specimens were obtained from 41 SS patients (10 with lymphoma) and 23 patients with SLE, respectively. PBMC and sera were also collected from 73 SLE patients. Full-length L1 transcripts, members of the APOBEC and IFN family were quantitated by real time PCR. Type I IFN activity was assessed in lupus plasma by a cell assay.

RESULTS: APOBEC3A was increased in SS MSG, SLE KB and PBMC and correlated with L1. AID and APOBEC3G were particularly overexpressed in MSG tissues derived from SS lymphoma patients.

CONCLUSION: These data reveal a previously unappreciated role of APOBEC family proteins in the pathogenesis of systemic autoimmunity and SS related lymphomagenesis.

DOI10.1016/j.clim.2020.108649
Alternate JournalClin Immunol
PubMed ID33326823
Grant ListR01 AI059893 / AI / NIAID NIH HHS / United States
Related Faculty: 
Surya V. Seshan, M.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700