Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates.

TitleEvaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates.
Publication TypeJournal Article
Year of Publication2016
AuthorsArnold AR, Burnham C-AD, Ford BA, Lawhon SD, McAllister SK, Lonsway D, Albrecht V, Jerris RC, Rasheed JK, Limbago B, Burd EM, Westblade LF
JournalJ Clin Microbiol
Volume54
Issue3
Pagination745-8
Date Published2016 Mar
ISSN1098-660X
KeywordsAnimals, Chromatography, Affinity, Humans, Penicillin-Binding Proteins, Peptide Synthases, Reproducibility of Results, Sensitivity and Specificity, Staphylococcal Infections, Staphylococcus intermedius, Staphylococcus lugdunensis
Abstract

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.

DOI10.1128/JCM.02869-15
Alternate JournalJ Clin Microbiol
PubMed ID26677248
PubMed Central IDPMC4767977
Related Faculty: 
Lars Westblade, Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700