An epitope within the DNA-binding domain of the herpes simplex virus immediate early protein Vmw175 is conserved in the varicella-zoster virus gene 62 protein.

TitleAn epitope within the DNA-binding domain of the herpes simplex virus immediate early protein Vmw175 is conserved in the varicella-zoster virus gene 62 protein.
Publication TypeJournal Article
Year of Publication1993
AuthorsEverett R, Cross A, Tyler J, Orr A
JournalJ Gen Virol
Volume74 ( Pt 9)
Pagination1955-8
Date Published1993 Sep
ISSN0022-1317
KeywordsAmino Acid Sequence, Animals, Antibodies, Monoclonal, Conserved Sequence, DNA-Binding Proteins, Epitopes, Herpesvirus 3, Human, Mice, Molecular Sequence Data, Recombinant Proteins, Sequence Homology, Amino Acid, Simplexvirus, Viral Proteins
Abstract

We have isolated a panel of monoclonal antibodies that recognize the DNA-binding domain of the herpes simplex virus type 1 (HSV-1) immediate early polypeptide Vmw175. The mice used for the fusions had been immunized with the isolated Vmw175 DNA-binding domain. This had been purified from bacteria that carried a phage T7 expression plasmid with the DNA-binding domain coding region. The epitopes recognized by the monoclonal antibodies were mapped by using a family of truncated versions of the DNA-binding domain, which had also been expressed in the bacterial expression system. The monoclonal antibodies divided into at least four different groups according to this mapping. Several of the monoclonal antibodies recognized Vmw175 expressed in infected BHK cells by HSV-1 strain 17 in Western blots. One of them also recognized the corresponding protein of varicella-zoster virus gene 62. This is further illustration of the relatedness of the two polypeptides.

DOI10.1099/0022-1317-74-9-1955
Alternate JournalJ Gen Virol
PubMed ID7690843
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