Title | Epigenetic inheritance of an inducibly nucleosome-depleted promoter and its associated transcriptional state in the apparent absence of transcriptional activators. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Ohsawa R, Adkins M, Tyler JK |
Journal | Epigenetics Chromatin |
Volume | 2 |
Issue | 1 |
Pagination | 11 |
Date Published | 2009 Sep 11 |
ISSN | 1756-8935 |
Abstract | BACKGROUND: Dynamic changes to the chromatin structure play a critical role in transcriptional regulation. This is exemplified by the Spt6-mediated histone deposition on to histone-depleted promoters that results in displacement of the general transcriptional machinery during transcriptional repression. RESULTS: Using the yeast PHO5 promoter as a model, we have previously shown that blocking Spt6-mediated histone deposition on to the promoter leads to persistent transcription in the apparent absence of transcriptional activators in vivo. We now show that the nucleosome-depleted PHO5 promoter and its associated transcriptionally active state can be inherited through DNA replication even in the absence of transcriptional activators. Transcriptional reinitiation from the nucleosome-depleted PHO5 promoter in the apparent absence of activators in vivo does not require Mediator. Notably, the epigenetic inheritance of the nucleosome-depleted PHO5 promoter through DNA replication does not require ongoing transcription. CONCLUSION: Our results suggest that there may be a memory or an epigenetic mark on the nucleosome-depleted PHO5 promoter that is independent of the transcription apparatus and maintains the promoter in a nucleosome-depleted state through DNA replication. |
DOI | 10.1186/1756-8935-2-11 |
Alternate Journal | Epigenetics Chromatin |
PubMed ID | 19747370 |
Related Faculty:
Jessica K. Tyler, Ph.D.