|Title||Dye-free spectrophotometric measurement of nucleic acid-to-protein ratio for cell-selective extracellular vesicle discrimination.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Sun D, Zhao Z, Spiegel S, Liu Y, Fan J, Amrollahi P, Hu J, Lyon CJ, Wan M, Hu TY|
|Date Published||2021 May 01|
|Keywords||Biomarkers, Biosensing Techniques, Extracellular Vesicles, Humans, Nucleic Acids, Proteins|
Extracellular vesicles (EVs) can represent a novel source of disease biomarkers, and are under intensive study for their clinical potential. Most EV-based cancer diagnostic studies have focused on establishing EV assays that detect increased expression of a single cancer-associated marker or marker signatures based on multiplex detection of these biomarkers. EV biomarker readouts can be obscured by high background signal leading to false positives, and may markedly differ between analyses due to variation in sample purity during EV isolation. This can obstruct the comparisons among studies and lead to conflicting conclusions. This work reports that the nucleic acid to protein UV absorption ratio of an EV is a cell-specific EV characteristic. This EV collective attribute can be measured at low-cost to discriminate EVs derived from malignant and non-malignant cells rather than employing single markers that may be cancer- or subtype-specific. Our work also highlighted the application for accessing purity in EV preparations irrelevant to EV yield. It can be employed to distinguish from patients with and without malignant disease upon analysis of EVs isolated from their serum samples.
|Alternate Journal||Biosens Bioelectron|
|PubMed Central ID||PMC7995647|
|Grant List||R21 AI126361 / AI / NIAID NIH HHS / United States |
R01 AI113725 / AI / NIAID NIH HHS / United States
R01 HD090927 / HD / NICHD NIH HHS / United States
R01 AI122932 / AI / NIAID NIH HHS / United States
R03 CA252783 / CA / NCI NIH HHS / United States
Zhen Zhao, Ph.D.