Title | Development of Resistance to EGFR-Targeted Therapy in Malignant Glioma Can Occur through EGFR-Dependent and -Independent Mechanisms. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Klingler S, Guo B, Yao J, Yan H, Zhang L, Vaseva AV, Chen S, Canoll P, Horner JW, Y Wang A, Paik J-H, Ying H, Zheng H |
Journal | Cancer Res |
Volume | 75 |
Issue | 10 |
Pagination | 2109-19 |
Date Published | 2015 May 15 |
ISSN | 1538-7445 |
Keywords | Animals, Brain Neoplasms, Crizotinib, Cyclin-Dependent Kinase Inhibitor p16, Doxycycline, Drug Resistance, Neoplasm, ErbB Receptors, Erlotinib Hydrochloride, Gefitinib, Glioma, Humans, Imidazoles, Mice, Inbred C57BL, Mice, Transgenic, Molecular Targeted Therapy, Phosphorylation, Protein Processing, Post-Translational, PTEN Phosphohydrolase, Pyrazoles, Pyridines, Quinazolines, Quinolines, Tumor Cells, Cultured |
Abstract | Epidermal growth factor receptor (EGFR) is highly amplified, mutated, and overexpressed in human malignant gliomas. Despite its prevalence and growth-promoting functions, therapeutic strategies to inhibit EGFR kinase activity have not been translated into profound beneficial effects in glioma clinical trials. To determine the roles of oncogenic EGFR signaling in gliomagenesis and tumor maintenance, we generated a novel glioma mouse model driven by inducible expression of a mutant EGFR (EGFR*). Using combined genetic and pharmacologic interventions, we revealed that EGFR*-driven gliomas were insensitive to EGFR tyrosine kinase inhibitors, although they could efficiently inhibit EGFR* autophosphorylation in vitro and in vivo. This is in contrast with the genetic suppression of EGFR* induction that led to significant tumor regression and prolonged animal survival. However, despite their initial response to genetic EGFR* extinction, all tumors would relapse and propagate independent of EGFR*. We further showed that EGFR*-independent tumor cells existed prior to treatment and were responsible for relapse following genetic EGFR* suppression. And, the addition of a PI3K/mTOR inhibitor could significantly delay relapse and prolong animal survival. Our findings shed mechanistic insight into EGFR drug resistance in glioma and provide a platform to test therapies targeting aberrant EGFR signaling in this setting. |
DOI | 10.1158/0008-5472.CAN-14-3122 |
Alternate Journal | Cancer Res |
PubMed ID | 25808866 |
PubMed Central ID | PMC4433602 |
Grant List | P30 CA045508 / CA / NCI NIH HHS / United States P50 CA127001 / CA / NCI NIH HHS / United States CA45508 / CA / NCI NIH HHS / United States P50CA127001 / CA / NCI NIH HHS / United States |
Related Lab:
Related Faculty:
Hongwu Zheng, Ph.D. Ji-Hye Paik, Ph.D.