Title | Detection of Kaposi's sarcoma associated herpesvirus nucleic acids using a smartphone accessory. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Mancuso M, Cesarman E, Erickson D |
Journal | Lab Chip |
Volume | 14 |
Issue | 19 |
Pagination | 3809-16 |
Date Published | 2014 Oct 07 |
ISSN | 1473-0189 |
Keywords | Cell Phone, DNA, Viral, Equipment Design, Gold, Herpesvirus 8, Human, Humans, Metal Nanoparticles, Microfluidic Analytical Techniques, Point-of-Care Systems, Sarcoma, Kaposi |
Abstract | Kaposi's sarcoma (KS) is an infectious cancer occurring in immune-compromised patients, caused by Kaposi's sarcoma associated herpesvirus (KSHV). Our vision is to simplify the process of KS diagnosis through the creation of a smartphone based point-of-care system capable of yielding an actionable diagnostic readout starting from a raw biopsy sample. In this work we develop the sensing mechanism for the overall system, a smartphone accessory capable of detecting KSHV nucleic acids. The accessory reads out microfluidic chips filled with a colorimetric nanoparticle assay targeted at KSHV. We calculate that our final device can read out gold nanoparticle solutions with an accuracy of 0.05 OD, and we demonstrate that it can detect DNA sequences from KSHV down to 1 nM. We believe that through integration with our previously developed components, a smartphone based system like the one studied here can provide accurate detection information, as well as a simple platform for field based clinical diagnosis and research. |
DOI | 10.1039/c4lc00517a |
Alternate Journal | Lab Chip |
PubMed ID | 25117534 |
Grant List | EB016803 / EB / NIBIB NIH HHS / United States |
Related Faculty:
Ethel Cesarman, M.D., Ph.D.