Detection of Kaposi's sarcoma associated herpesvirus nucleic acids using a smartphone accessory.

TitleDetection of Kaposi's sarcoma associated herpesvirus nucleic acids using a smartphone accessory.
Publication TypeJournal Article
Year of Publication2014
AuthorsMancuso M, Cesarman E, Erickson D
JournalLab Chip
Volume14
Issue19
Pagination3809-16
Date Published2014 Oct 07
ISSN1473-0189
KeywordsCell Phone, DNA, Viral, Equipment Design, Gold, Herpesvirus 8, Human, Humans, Metal Nanoparticles, Microfluidic Analytical Techniques, Point-of-Care Systems, Sarcoma, Kaposi
Abstract

Kaposi's sarcoma (KS) is an infectious cancer occurring in immune-compromised patients, caused by Kaposi's sarcoma associated herpesvirus (KSHV). Our vision is to simplify the process of KS diagnosis through the creation of a smartphone based point-of-care system capable of yielding an actionable diagnostic readout starting from a raw biopsy sample. In this work we develop the sensing mechanism for the overall system, a smartphone accessory capable of detecting KSHV nucleic acids. The accessory reads out microfluidic chips filled with a colorimetric nanoparticle assay targeted at KSHV. We calculate that our final device can read out gold nanoparticle solutions with an accuracy of 0.05 OD, and we demonstrate that it can detect DNA sequences from KSHV down to 1 nM. We believe that through integration with our previously developed components, a smartphone based system like the one studied here can provide accurate detection information, as well as a simple platform for field based clinical diagnosis and research.

DOI10.1039/c4lc00517a
Alternate JournalLab Chip
PubMed ID25117534
Grant ListEB016803 / EB / NIBIB NIH HHS / United States
Related Faculty: 
Ethel Cesarman, M.D., Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700