Title | Combined double CK5/P63 stain: useful adjunct test for diagnosing pulmonary squamous cell carcinoma. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Fatima N, Cohen C, Lawson D, Siddiqui MT |
Journal | Diagn Cytopathol |
Volume | 40 |
Issue | 11 |
Pagination | 943-8 |
Date Published | 2012 Nov |
ISSN | 1097-0339 |
Keywords | Adenocarcinoma, Adenocarcinoma of Lung, Antigens, Neoplasm, Biomarkers, Tumor, Biopsy, Fine-Needle, Carcinoma, Squamous Cell, Cohort Studies, Hematoxylin, Humans, Immunohistochemistry, Keratin-5, Lung, Lung Neoplasms, Membrane Proteins, Reproducibility of Results, Sensitivity and Specificity, Staining and Labeling |
Abstract | Increasing demand for accurate differentiation of pulmonary squamous cell carcinoma (SQCC) from other subtypes can be challenging for pathologists. This is more so in fine-needle aspirations (FNA) since the sample is small and SQCC may show degenerative changes and necrosis that distort the cellular features. Immunohistochemistry (IHC) is a valuable adjunct, and CK5/6 and P63 immunoreactivity is found to be basically restricted to SQCC. In our study, we evaluated the efficiency of CK5/P63 double staining in the diagnosis of pulmonary SQCC in cell blocks (CB) of lung FNA. We used a cohort including 24 CB of lung SQCC and 34 CB of lung adenocarcinomas (ADC). IHC was performed for CK5/P63 double stain. Seventeen of 24 (70%) lung SQCC were positive for the double stain CK5/P63. Two (8%) were positive for CK5 alone and two (8%) were positive for P63 alone. Thus, a total 19 of 24(79%) SQCC of the lung were positive for CK5 and P63 each. In ADC, no immunoreactivity was detected for CK5 alone or combined CK5/P63. Three of 34(8%) ADC were positive for P63. This first study of double staining of CK5/P63 in FNA CB shows a sensitivity of 70% and specificity of 100% for SQCC of the lung. When each marker staining alone is included, the sensitivity for CK5 and P63 increases to 79% each. This double stain can help in the diagnosis of pulmonary SQCC with an accuracy of 88% and a positive predictive value of 100%. |
DOI | 10.1002/dc.21678 |
Alternate Journal | Diagn Cytopathol |
PubMed ID | 21472873 |
Related Faculty:
Momin Siddiqui, M.D.