| Title | Ca2+-induced changes in the secondary structure of a 60 kDa phosphoinositide-specific phospholipase C from bovine brain cytosol. |
| Publication Type | Journal Article |
| Year of Publication | 1988 |
| Authors | Herrero C, Cornet ME, Lopez C, Barreno PG, Municio AM, Moscat J |
| Journal | Biochem J |
| Volume | 255 |
| Issue | 3 |
| Pagination | 807-12 |
| Date Published | 1988 Nov 01 |
| ISSN | 0264-6021 |
| Keywords | Animals, Brain, Calcium, Cattle, Cell Line, Cytosol, Enzyme Activation, Phosphatidylinositol Diacylglycerol-Lyase, Phosphoric Diester Hydrolases, Protein Binding, Protein Conformation |
| Abstract | The purification to homogeneity of a 60 kDa phosphoinositide-specific phospholipase C from bovine brain cytosol is reported here. This enzyme exhibits the same properties, in terms of response to Ca2+, as does the cytosolic activity in a variety of cell types. We show here that Ca2+ does not appear to modulate the binding of the enzyme to the substrate, but induces dramatic changes in its secondary structure. Therefore we suggest that a decrease in the alpha-helix content of this enzyme correlates with its ability to be activated by Ca2+. |
| DOI | 10.1042/bj2550807 |
| Alternate Journal | Biochem J |
| PubMed ID | 2850798 |
| PubMed Central ID | PMC1135313 |
Related Faculty:
Jorge Moscat, Ph.D.