Title | Blood-Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Carbapenemase-Producing Directly from Positive Blood Culture Broths. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Sfeir MM, Satlin MJ, Fauntleroy KA, Jenkins SG, Westblade LF |
Journal | J Clin Microbiol |
Volume | 58 |
Issue | 2 |
Date Published | 2020 01 28 |
ISSN | 1098-660X |
Keywords | Anti-Bacterial Agents, Bacterial Proteins, beta-Lactamases, Blood Culture, Carbapenem-Resistant Enterobacteriaceae, Carbapenems, Enterobacteriaceae Infections, Humans, Inactivation, Metabolic, Microbial Sensitivity Tests, Phenotype, Reproducibility of Results, Sensitivity and Specificity |
Abstract | A variant of the modified carbapenem inactivation method (mCIM) was developed to detect carbapenemase activity directly from positive blood culture broths. The method, termed "Blood-mCIM," was evaluated using Bactec blood culture bottles (Becton, Dickinson and Company, Franklin Lakes, NJ) inoculated with 27 different carbapenemase-producing (CPE) isolates and 34 different non-CPE isolates. The assay was positive for all blood culture broths inoculated with CPE isolates and negative for all blood culture broths inoculated with non-CPE isolates, corresponding to a diagnostic sensitivity and specificity of 100%, respectively. This assay is inexpensive using "off the shelf" reagents, does not require centrifugation or mechanical lysis, and can be readily implemented in any clinical microbiology laboratory. The Blood-mCIM should facilitate expedient administration of antimicrobial therapy targeted toward CPE bloodstream infections and assist infection control and public health surveillance. |
DOI | 10.1128/JCM.01377-19 |
Alternate Journal | J Clin Microbiol |
PubMed ID | 31748319 |
PubMed Central ID | PMC6989087 |
Related Faculty:
Lars Westblade, Ph.D.