Title | Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Wike CL, Graves HK, Hawkins R, Gibson MD, Ferdinand MB, Zhang T, Chen Z, Hudson DF, Ottesen JJ, Poirier MG, Schumacher J, Tyler JK |
Journal | Elife |
Volume | 5 |
Pagination | e11402 |
Date Published | 2016 Feb 16 |
ISSN | 2050-084X |
Keywords | Adenosine Triphosphatases, Animals, Aurora Kinase A, Cell Cycle Proteins, Cell Line, Chromosomal Proteins, Non-Histone, DNA, DNA-Binding Proteins, Drosophila, Histones, Humans, Mitosis, Multiprotein Complexes, Phosphorylation, Protein Processing, Post-Translational, Threonine |
Abstract | Phosphorylation of histone H3 threonine 118 (H3 T118ph) weakens histone DNA-contacts, disrupting the nucleosome structure. We show that Aurora-A mediated H3 T118ph occurs at pericentromeres and chromosome arms during prophase and is lost upon chromosome alignment. Expression of H3 T118E or H3 T118I (a SIN mutation that bypasses the need for the ATP-dependent nucleosome remodeler SWI/SNF) leads to mitotic problems including defects in spindle attachment, delayed cytokinesis, reduced chromatin packaging, cohesion loss, cohesin and condensin I loss in human cells. In agreement, overexpression of Aurora-A leads to increased H3 T118ph levels, causing cohesion loss, and reduced levels of cohesin and condensin I on chromatin. Normal levels of H3 T118ph are important because it is required for development in fruit flies. We propose that H3 T118ph alters the chromatin structure during specific phases of mitosis to promote timely condensin I and cohesin disassociation, which is essential for effective chromosome segregation. |
DOI | 10.7554/eLife.11402 |
Alternate Journal | Elife |
PubMed ID | 26878753 |
PubMed Central ID | PMC4798946 |
Grant List | P30 CA016672 / CA / NCI NIH HHS / United States R01 GM083055 / GM / NIGMS NIH HHS / United States R01 CA95641 / CA / NCI NIH HHS / United States R01GM64475 / GM / NIGMS NIH HHS / United States R01 GM064475 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Jessica K. Tyler, Ph.D.