| Title | Assessment of the Utility of Cytology and Flow Cytometry of Cerebrospinal Fluid Samples in Clinical Practice. |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Nam AS, Giorgadze T, Tam W, Chadburn A |
| Journal | Acta Cytol |
| Volume | 62 |
| Issue | 2 |
| Pagination | 130-136 |
| Date Published | 2018 |
| ISSN | 1938-2650 |
| Keywords | Adolescent, Adult, Aged, Aged, 80 and over, Cerebrospinal Fluid, Child, Child, Preschool, Cytological Techniques, Female, Flow Cytometry, Hematologic Neoplasms, Humans, Male, Meningeal Neoplasms, Middle Aged, Predictive Value of Tests, Reproducibility of Results, Retrospective Studies, Young Adult |
| Abstract | OBJECTIVES: We sought to assess the utility and limitations of both flow cytometry (FC) and cytology for the analysis of cerebrospinal fluid (CSF) in a practical clinical setting. METHODS: A total of 393 consecutive CSF samples from 171 patients submitted for both cytomorphologic and FC assessments were analyzed. RESULTS: Both FC and cytology findings were negative for malignancy in 315/393 samples (80%), and either positive (POS) or suspicious/atypical (SUSP/AT) in 7% of samples. This resulted in high agreement between FC and cytology (87%). Minor discrepancies were present in 4% of the cases. In 28 samples, an abnormal population was detected by FC but not by cytology. CONCLUSIONS: FC and cytology are important complementary methods for analyzing CSF samples. In cases where cytology is SUSP/AT and FC is inconclusive or negative, additional specimens should be submitted for immunostaining, cytogenetics, and/or molecular studies. |
| DOI | 10.1159/000487070 |
| Alternate Journal | Acta Cytol |
| PubMed ID | 29510385 |
Related Faculty:
Amy Chadburn, M.D.