| Title | Assessment of Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Bacterial and Yeast Identification. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Westblade LF, Garner OB, MacDonald K, Bradford C, Pincus DH, A Mochon B, Jennemann R, Manji R, Bythrow M, Lewinski MA, Burnham C-AD, Ginocchio CC |
| Journal | J Clin Microbiol |
| Volume | 53 |
| Issue | 7 |
| Pagination | 2349-52 |
| Date Published | 2015 Jul |
| ISSN | 1098-660X |
| Keywords | Bacteria, Humans, Microbiological Techniques, Reproducibility of Results, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Yeasts |
| Abstract | Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification. |
| DOI | 10.1128/JCM.00187-15 |
| Alternate Journal | J Clin Microbiol |
| PubMed ID | 25926486 |
| PubMed Central ID | PMC4473194 |
Related Faculty:
Lars Westblade, Ph.D.