Title | Assessment of HER-2/neu status in breast cancer. Automated Cellular Imaging System (ACIS)-assisted quantitation of immunohistochemical assay achieves high accuracy in comparison with fluorescence in situ hybridization assay as the standard. |
Publication Type | Journal Article |
Year of Publication | 2001 |
Authors | Wang S, Saboorian MH, Frenkel EP, Haley BB, Siddiqui MT, Gokaslan S, Wians FH, Hynan L, Ashfaq R |
Journal | Am J Clin Pathol |
Volume | 116 |
Issue | 4 |
Pagination | 495-503 |
Date Published | 2001 Oct |
ISSN | 0002-9173 |
Keywords | Breast Neoplasms, Gene Amplification, Gene Expression, Humans, Image Processing, Computer-Assisted, Immunohistochemistry, In Situ Hybridization, Fluorescence, Receptor, ErbB-2, Retrospective Studies, ROC Curve, Sensitivity and Specificity |
Abstract | This retrospective study of formalin-fixed infiltrating breast cancer specimens compared manual immunohistochemical assay with a new image analyzer-assisted immunohistochemical quantitation method, using fluorescence in situ hybridization assay (FISH) as the standard. Following the manual immunohistochemical assay, 189 cases, including most manual immunohistochemically positive and some random negative cases, were analyzed by FISH assay for Her-2/neu gene amplification and by the Automated Cellular Imaging System (ACIS) for immunohistochemical staining. Using the FISH standard, the ACIS immunohistochemical assay attained a higher concordance rate and sensitivity than the manual immunohistochemical assay (91.0% and 88% vs 85.7% and 71%, respectively), with only a slight decrease in specificity (93% vs 96%, respectively). In particular, the ACIS immunohistochemical assay resulted in a higher correlation with the FISH assay in the manual immunohistochemical assay 2+ cases. The ACIS immunohistochemical assay achieved higher accuracy than the manual method according to receiver operating characteristic curve analysis. The ACIS method represents a substantial improvement over the manual method for objective evaluation of the HER-2/neu status. |
DOI | 10.1309/TMUW-G4WB-LXJ2-FUDN |
Alternate Journal | Am J Clin Pathol |
PubMed ID | 11601134 |
Related Faculty:
Momin Siddiqui, M.D.