Title | Wnt signaling though beta-catenin is required for prostate lineage specification. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Simons BW, Hurley PJ, Huang Z, Ross AE, Miller R, Marchionni L, Berman DM, Schaeffer EM |
Journal | Dev Biol |
Volume | 371 |
Issue | 2 |
Pagination | 246-55 |
Date Published | 2012 Nov 15 |
ISSN | 1095-564X |
Keywords | Animals, beta Catenin, Cell Differentiation, Homeodomain Proteins, Male, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Prostate, Transcription Factors, Wnt Signaling Pathway |
Abstract | Androgens initiate a complex network of signals within the UGS that trigger prostate lineage commitment and bud formation. Given its contributions to organogenesis in other systems, we investigated a role for canonical Wnt signaling in prostate development. We developed a new method to achieve complete deletion of beta-catenin, the transcriptional coactivator required for canonical Wnt signaling, in early prostate development. Beta-catenin deletion abrogated canonical Wnt signaling and yielded prostate rudiments that exhibited dramatically decreased budding and failed to adopt prostatic identity. This requirement for canonical Wnt signaling was limited to a brief critical period during the initial molecular phase of prostate identity specification. Deletion of beta-catenin in the adult prostate did not significantly affect organ homeostasis. Collectively, these data establish that beta-catenin and Wnt signaling play key roles in prostate lineage specification and bud outgrowth. |
DOI | 10.1016/j.ydbio.2012.08.016 |
Alternate Journal | Dev Biol |
PubMed ID | 22960283 |
Grant List | P30CA006973 / CA / NCI NIH HHS / United States DK081019 / DK / NIDDK NIH HHS / United States / HHMI / Howard Hughes Medical Institute / United States 5R01DK72000-5 / DK / NIDDK NIH HHS / United States |
Related Faculty:
Luigi Marchionni, M.D., Ph.D.