In vitro generated human monoclonal trinitrophenyl-specific B cell lines. Evidence that human and murine anti-trinitrophenyl monoclonal antibodies cross-react with Escherichia coli beta-galactosidase.

TitleIn vitro generated human monoclonal trinitrophenyl-specific B cell lines. Evidence that human and murine anti-trinitrophenyl monoclonal antibodies cross-react with Escherichia coli beta-galactosidase.
Publication TypeJournal Article
Year of Publication1987
AuthorsGolding B, Inghirami G, Peters E, Hoffman T, Balow JE, Tsokos GC
JournalJ Immunol
Volume139
Issue12
Pagination4061-6
Date Published1987 Dec 15
ISSN0022-1767
KeywordsAnimals, Antibodies, Monoclonal, B-Lymphocytes, Bacterial Proteins, beta-Galactosidase, Cell Line, Cell Transformation, Viral, Cross Reactions, Escherichia coli, Galactosidases, Herpesvirus 4, Human, Humans, Immunoglobulin kappa-Chains, Immunoglobulin M, Mice, Nitrobenzenes, Trinitrobenzenes
Abstract

Stable human antigen-specific monoclonal B cell lines were established without prior in vivo immunization. This was accomplished by expanding the anti-trinitrophenyl (TNP) B cells in vitro with the antigen TNP-Brucella abortus and then immortalizing them with Epstein-Barr virus. Five anti-TNP clones were selected by sequential limiting dilution. All five anti-TNP clones secreted IgM kappa antibodies. When tested against a panel of self and environmental antigens, all five anti-TNP clones exhibited cross-reactivity with an Escherichia coli-derived beta-galactosidase. To determine whether this was a more general phenomenon, a panel of murine monoclonals were tested and found to bind to beta-galactosidase. It is therefore possible that human and murine anti-TNP beta cell responses reflect reactivity against an environmental antigen, namely an epitope present on E. coli-derived beta-galactosidase. This approach of expanding human antigen-specific B cells by antigen stimulation in vitro, with a T-independent hapten-carrier conjugate before Epstein-Barr virus transformation, may prove useful in the development of human monoclonals for therapeutic purposes.

Alternate JournalJ Immunol
PubMed ID2826579
Related Faculty: 
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