| Title | The transcriptional landscape of the mammalian genome. |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, Maeda N, Oyama R, Ravasi T, Lenhard B, Wells C et al. |
| Corporate Authors | FANTOM Consortium, RIKEN Genome Exploration Research Group and Genome Science Group(Genome Network Project Core Group) |
| Journal | Science |
| Volume | 309 |
| Issue | 5740 |
| Pagination | 1559-63 |
| Date Published | 2005 Sep 02 |
| ISSN | 1095-9203 |
| Keywords | 3' Untranslated Regions, Animals, Base Sequence, Conserved Sequence, DNA, Complementary, Genome, Genome, Human, Genomics, Humans, Mice, Promoter Regions, Genetic, Proteins, Regulatory Sequences, Ribonucleic Acid, RNA, RNA Splicing, RNA, Untranslated, Terminator Regions, Genetic, Transcription Initiation Site, Transcription, Genetic |
| Abstract | This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. |
| DOI | 10.1126/science.1112014 |
| Alternate Journal | Science |
| PubMed ID | 16141072 |
| Grant List | TGM03P17 / / Telethon / Italy TGM06S01 / / Telethon / Italy |
Related Faculty:
Luigi Marchionni, M.D., Ph.D.