| Title | TRAF6 and p62 inhibit amyloid β-induced neuronal death through p75 neurotrophin receptor. |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Geetha T, Zheng C, McGregor WC, B White D, Diaz-Meco MT, Moscat J, Babu JRamesh |
| Journal | Neurochem Int |
| Volume | 61 |
| Issue | 8 |
| Pagination | 1289-93 |
| Date Published | 2012 Dec |
| ISSN | 1872-9754 |
| Keywords | Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Amyloid beta-Peptides, Animals, Apoptosis, Cell Line, DNA, Antisense, Heat-Shock Proteins, Hippocampus, Humans, Mice, Molecular Sequence Data, Nerve Growth Factor, Neurons, NF-kappa B, Peptide Fragments, Protein Processing, Post-Translational, Receptors, Nerve Growth Factor, Recombinant Fusion Proteins, Sequence Deletion, Sequestosome-1 Protein, TNF Receptor-Associated Factor 6, Transfection, Ubiquitination |
| Abstract | Amyloid β (Aβ) aggregates are the primary component of senile plaques in Alzheimer disease (AD) patient's brain. Aβ is known to bind p75 neurotrophin receptor (p75(NTR)) and mediates Aβ-induced neuronal death. Recently, we showed that NGF leads to p75(NTR) polyubiquitination, which promotes neuronal cell survival. Here, we demonstrate that Aβ stimulation impaired the p75(NTR) polyubiquitination. TRAF6 and p62 are required for polyubiquitination of p75(NTR) on NGF stimulation. Interestingly, we found that overexpression of TRAF6/p62 restored p75(NTR) polyubiquitination upon Aβ/NGF treatment. Aβ significantly reduced NF-κB activity by attenuating the interaction of p75(NTR) with IKKβ. p75(NTR) increased NF-κB activity by recruiting TRAF6/p62, which thereby mediated cell survival. These findings indicate that TRAF6/p62 abrogated the Aβ-mediated inhibition of p75(NTR) polyubiquitination and restored neuronal cell survival. |
| DOI | 10.1016/j.neuint.2012.09.005 |
| Alternate Journal | Neurochem Int |
| PubMed ID | 23017601 |
Related Faculty:
Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.