Total lymphoid irradiation, without intrathymic injection of donor cells, induces indefinite acceptance of heart but not islet or skin allografts in rats.

TitleTotal lymphoid irradiation, without intrathymic injection of donor cells, induces indefinite acceptance of heart but not islet or skin allografts in rats.
Publication TypeJournal Article
Year of Publication1996
AuthorsPapalois BE, Wahoff DC, Aasheim TC, Griffin RJ, Jessurun J, Clemmings SM, Field JM, Leone JP, Sutherland DE
JournalTranspl Int
Volume9 Suppl 1
PaginationS372-8
Date Published1996
ISSN0934-0874
KeywordsAnimals, Cell Transplantation, Graft Survival, Heart Transplantation, Immune Tolerance, Islets of Langerhans Transplantation, Lymphatic Irradiation, Male, Rats, Rats, Inbred Lew, Rats, Inbred WF, Skin Transplantation, Spleen, Thymus Gland, Transplantation, Homologous
Abstract

Allograft tolerance occurs in rodents given a dose of antilymphocyte serum (ALS) and intrathymic injection (ITI) of donor splenocytes (SC) 1-3 weeks prior to transplant (TX). The purpose of our study was to test total lymphoid irradiation (TLI) as an alternative to ALS in ITI tolerance induction to heart, islet, and skin allografts. Prepubertal Wistar Furth rats were recipients. ITI of donor (Lewis) SC was done at the end of the TLI course. Rats received either a heterotopic heart, a skin graft, or 2300 islets (diabetic recipients) intraportally from Lewis donors. TLI (without ITI) in a dose of 200 rads/day for 5 consecutive days, followed by TX in 3 weeks resulted in indefinite acceptance of heart (but not islet or skin) grafts in 60% of the recipients. These data indicate that TLI by a dose schedule of 200 rads/day for 5 days should be tested for clinical relevance in large animal recipients of immediately vascularized grafts.

DOI10.1007/978-3-662-00818-8_91
Alternate JournalTranspl Int
PubMed ID8959867
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