Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.

TitleStromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium.
Publication TypeJournal Article
Year of Publication1994
AuthorsOsteen KG, Rodgers WH, Gaire M, Hargrove JT, Gorstein F, Matrisian LM
JournalProc Natl Acad Sci U S A
Date Published1994 Oct 11
KeywordsCell Communication, Cells, Cultured, Embryo Implantation, Endometrium, Enzyme Precursors, Epithelium, Estradiol, Female, Gene Expression, Humans, In Situ Hybridization, Matrix Metalloproteinase 3, Matrix Metalloproteinase 7, Menstrual Cycle, Metalloendopeptidases, Ovulation, Progesterone, Transcription, Genetic

The hallmark of the menstrual cycle is extensive steroid-dependent tissue turnover. Estrogen mediates endometrial cell growth and structural remodeling, whereas progesterone suppresses estrogen-dependent proliferation and promotes cellular differentiation. In nonfertile cycles, tissue degradation and menstruation occur as a consequence of steroidal deprivation as the ovarian corpus luteum fails. Stromal-epithelial interactions are recognized as a necessary component in mediating steroid-induced endometrial turnover. Specific mRNAs for metalloproteinases of the stromelysin family are expressed during endometrial growth and menstrual breakdown but are absent in the progestin-dominated secretory phase. This expression pattern suggests involvement of stromelysins in remodeling the extracellular matrix of the endometrium during tissue growth and breakdown and implicates progesterone in the suppression of these enzymes. We examined the regulation of endometrial stromelysins in explant cultures and found no acute effect of estradiol on their expression, whereas progesterone was a potent inhibitor of stromelysin expression. Progesterone also suppressed stromelysin expression in cultures of isolated stromal cells, but epithelial cells were progesterone insensitive. Coculture of recombined stromal and epithelial cells restored steroidal suppression of the epithelial-specific metalloproteinase. Our data confirm that progesterone inhibits endometrial stromelysins and further demonstrate the necessity for a stromal-derived factor(s) as a mediator of steroid suppression of an epithelial metalloproteinase.

Alternate JournalProc Natl Acad Sci U S A
PubMed ID7937850
PubMed Central IDPMC44971
Grant ListCA46853 / CA / NCI NIH HHS / United States
HD28128 / HD / NICHD NIH HHS / United States
HD30472 / HD / NICHD NIH HHS / United States
Related Faculty: 
William Rodgers, M.D., Ph.D.

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