Recovery of prostacyclin production by de-endothelialized rabbit aorta. Critical role of neointimal smooth muscle cells.

TitleRecovery of prostacyclin production by de-endothelialized rabbit aorta. Critical role of neointimal smooth muscle cells.
Publication TypeJournal Article
Year of Publication1981
AuthorsEldor A, Falcone DJ, Hajjar DP, Minick CR, Weksler BB
JournalJ Clin Invest
Volume67
Issue3
Pagination735-41
Date Published1981 Mar
ISSN0021-9738
KeywordsAnimals, Aorta, Cell Differentiation, Endothelium, Epoprostenol, Microscopy, Electron, Muscle, Smooth, Vascular, Prostaglandins, Rabbits, Thrombosis, Time Factors
Abstract

Prostacyclin (PGI2) synthetic capacity was assayed at the surface of aortas at various intervals after removal of endothelium with a balloon catheter. Results were correlated with morphologic changes in the vessel wall seen by light microscopy, scanning and transmission electron microscopy. To assay PGI2 synthetic capacity, we applied an incubation chamber to the luminal surface of the aortas; after arachidonic acid stimulation we assayed the PGI2 synthesized with a bioassay and radioimmunoassay. PGI2 synthesis in de-endothelialized aortas was determined immediately after balloon-catheter injury and at intervals of 1 h and 2, 4, 15, 35, and 70 d. PGI2 synthesis was low at 1 h and increased over time with levels at 35 and 70 d reaching that of normal artery. Scanning and transmission electron microscopy of de-endothelialized areas showed persistent absence of endothelium with formation of a neointima composed of smooth muscle cells. De-endothelialized aorta was covered with adherent platelets shortly after injury, however several days later only a few platelets adhered to the denuded surface. Results indicated that (a) endothelium is responsible for nearly all PGI2 production at the luminal surface of the normal aorta, (b) de-endothelialized muscular neointima synthesized increasing quantities of PGI2 with time after injury, and (c) increase of PGI2 production at the luminal surface of de-endothelialized aorta correlates with formation of a neointima and with the acquired thromboresistance of the aorta.

DOI10.1172/JCI110090
Alternate JournalJ Clin Invest
PubMed ID7009648
PubMed Central IDPMC370624
Grant ListHL-01803 / HL / NHLBI NIH HHS / United States
HL-18828 / HL / NHLBI NIH HHS / United States
Related Faculty: 
David P. Hajjar, Ph.D. Domenick J. Falcone, Ph.D.

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