Quantitative proteomics reveal the protective effects of EDS against osteoarthritis via attenuating inflammation and modulating immune response.

TitleQuantitative proteomics reveal the protective effects of EDS against osteoarthritis via attenuating inflammation and modulating immune response.
Publication TypeJournal Article
Year of Publication2021
AuthorsHao Y, Wu Y, Wang S, Wang C, Qu S, Li L, Yu G, Liu Z, Zhao Z, Fan P, Zhang Z, Shi Y
JournalJ Ethnopharmacol
Date Published2021 May 10
KeywordsAnimals, Cartilage, Articular, Complement System Proteins, Cytokines, Disease Models, Animal, Drugs, Chinese Herbal, Immunity, Inflammation, Knee Joint, MAP Kinase Signaling System, Osteoarthritis, Pain Threshold, Papain, Proteome, Proteomics, Rats, Wistar, Ribosomal Proteins

ETHNOPHARMACOLOGICAL RELEVANCE: Epimedium brevicornu Maxim, Dioscorea nipponica Makino, and Salvia miltiorrhiza Bunge formula (EDS) are three traditional Chinese medicines commonly combined and used to treat osteoarthritis (OA). However, the mechanism of its therapeutic effect on OA is still unclear.

AIM OF THE STUDY: The aim of this study was to investigate the potential anti osteoarthritis mechanism of EDS in the treatment of OA rats' model by quantitative proteomics.

MATERIALS AND METHODS: A papain-induced rat OA model was established, and then EDS was intragastrically administered for 28 days. A label-free quantification proteomics was performed to evaluate the holistic efficacy of EDS against OA and identify the possible protein profiles mechanisms. The expression levels of critical changed proteins were validated by RT-qPCR and Western blotting. The effects of EDS were then assessed by evaluating pathologic changes in the affected knee joint and measuring pressure pain threshold, acoustic reflex threshold, angle of joint curvature.

RESULTS: Proteomics analysis showed that 62 proteins were significantly upregulated and 208 proteins were downregulated in OA group compared to control group. The changed proteins were involved in activation of humoral immunity response, complement cascade activation, leukocyte mediated immunity, acute inflammatory response, endocytosis regulation, and proteolysis regulation. The EDS treatment partially restored the protein profile changes. The protective effects of EDS on pathologic changes in OA rats' knee joint and pain threshold assessment were consisted with the proteomics results.

CONCLUSIONS: The results suggest that EDS exerted synergistic therapeutic efficacies to against OA through suppressing inflammation, modulating the immune system, relieving joint pain, and attenuating cartilage degradation.

Alternate JournalJ Ethnopharmacol
PubMed ID33421600
Related Faculty: 
Zhen Zhao, Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700