Protein kinase C zeta isoform is critical for mitogenic signal transduction.

TitleProtein kinase C zeta isoform is critical for mitogenic signal transduction.
Publication TypeJournal Article
Year of Publication1993
AuthorsBerra E, Diaz-Meco MT, Dominguez I, Municio MM, Sanz L, Lozano J, Chapkin RS, Moscat J
Date Published1993 Aug 13
Keywords3T3 Cells, Amino Acid Sequence, Animals, Bacillus cereus, Base Sequence, Cell Division, Cells, Cultured, DNA Replication, Female, Genetic Vectors, Growth Substances, Insulin, Isoenzymes, Mice, Molecular Sequence Data, Oligodeoxyribonucleotides, Oocytes, Phosphorylation, Plasmids, Protamine Kinase, Protein Kinase C, Proto-Oncogene Proteins p21(ras), RNA, Signal Transduction, Transfection, Type C Phospholipases, Xenopus laevis

The requirement of protein kinase C zeta (zeta PKC) for maturation of X. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase C has recently been shown. Here we present experimental evidence demonstrating that activation of zeta PKC is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. Furthermore, by using a dominant kinase-defective mutant of zeta PKC, we confirm that this kinase is required for mitogenic activation in oocytes and fibroblasts. These results permit us to propose zeta PKC as a critical step downstream of p21ras in mitogenic signal transduction.

Alternate JournalCell
PubMed ID7688666
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