| Title | Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: the MPAKT model. |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Majumder PK, Yeh JJen, George DJ, Febbo PG, Kum J, Xue Q, Bikoff R, Ma H, Kantoff PW, Golub TR, Loda M, Sellers WR |
| Journal | Proc Natl Acad Sci U S A |
| Volume | 100 |
| Issue | 13 |
| Pagination | 7841-6 |
| Date Published | 2003 Jun 24 |
| ISSN | 0027-8424 |
| Keywords | Animals, Enzyme Activation, Genotype, Humans, Immunoblotting, Immunohistochemistry, In Situ Hybridization, Male, Mice, Mice, Transgenic, Neovascularization, Pathologic, Oligonucleotide Array Sequence Analysis, Platelet Endothelial Cell Adhesion Molecule-1, Prostate, Prostatic Intraepithelial Neoplasia, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-akt, Ribosomal Protein S6 Kinases, 70-kDa, RNA, RNA, Messenger, Transgenes, Urinary Bladder |
| Abstract | To determine whether Akt activation was sufficient for the transformation of normal prostate epithelial cells, murine prostate restricted Akt kinase activity was generated in transgenic mice (MPAKT mice). Akt expression led to p70S6K activation, prostatic intraepithelial neoplasia (PIN), and bladder obstruction. mRNA expression profiles from MPAKT ventral prostate revealed similarities to human cancer and an angiogenic signature that included three angiogenin family members, one of which was found elevated in the plasma of men with prostate cancer. Thus, the MPAKT model may be useful in studying the role of Akt in prostate epithelial cell transformation and in the discovery of molecular markers relevant to human disease. |
| DOI | 10.1073/pnas.1232229100 |
| Alternate Journal | Proc Natl Acad Sci U S A |
| PubMed ID | 12799464 |
| PubMed Central ID | PMC164675 |
| Grant List | P01 CA089021 / CA / NCI NIH HHS / United States P01 CA89021 / CA / NCI NIH HHS / United States |
Related Faculty:
Massimo Loda, M.D.