| Title | ProEx C immunocytochemistry and high-risk human papillomavirus DNA testing in papanicolaou tests with atypical squamous cell (ASC-US) cytology: correlation study with histologic biopsy. |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Siddiqui MT, Hornaman K, Cohen C, Nassar A |
| Journal | Arch Pathol Lab Med |
| Volume | 132 |
| Issue | 10 |
| Pagination | 1648-52 |
| Date Published | 2008 Oct |
| ISSN | 1543-2165 |
| Keywords | Adolescent, Adult, Alphapapillomavirus, Antigens, Neoplasm, Biomarkers, Biopsy, Carcinoma, Squamous Cell, Cell Cycle Proteins, Cervix Uteri, DNA Topoisomerases, Type II, DNA, Viral, DNA-Binding Proteins, Female, Humans, Immunohistochemistry, Middle Aged, Minichromosome Maintenance Complex Component 2, Nuclear Proteins, Papanicolaou Test, Predictive Value of Tests, Retrospective Studies, Sensitivity and Specificity, Uterine Cervical Neoplasms, Vaginal Smears |
| Abstract | CONTEXT: Papanicolaou tests with atypical squamous cells of undetermined significance (ASC-US) cytology and adjunct testing for high-risk human papillomaviruses (hr-HPV) are helpful in detecting high-grade disease. Detection of disease may be further improved with molecular markers known to be overexpressed in cervical carcinoma. ProEx C detects 2 such molecular markers, minichromosome maintenance protein 2 and topoisomerase II, which are associated with abnormal cell cycle regulation. OBJECTIVE: To determine the utility of ProEx C as a marker for high-grade cervical intraepithelial neoplasia 2+ disease when compared with hr-HPV status in Papanicolaou tests with ASC-US cytology. DESIGN: A SurePath slide was prepared on all ASC-US cases from the residual SurePath vial pellet and stained using the ProEx C reagent prediluted with water-bath antigen retrieval, using a Dako autostainer. Nuclear staining of cytologically atypical squamous cells was considered a positive result. Adjunct testing for hr-HPV used Digene Hybrid Capture 2. Follow-up biopsy results were available for review following the Papanicolaou test. RESULTS: Two hundred patients with ASC-US diagnoses were part of this study. The sensitivities of ProEx C and hr-HPV testing in detecting high-grade cervical intraepithelial neoplasia 2+ disease were 98.04% and 82.35%, respectively, whereas the specificity for detecting high-grade disease was 74.50% and 73.15%, respectively. CONCLUSIONS: ProEx C staining is a more sensitive and specific biomarker for detecting cervical disease than adjunct testing for hr-HPV status in Papanicolaou tests with ASC-US. |
| DOI | 10.1043/1543-2165(2008)132[1648:PCIAHH]2.0.CO;2 |
| Alternate Journal | Arch Pathol Lab Med |
| PubMed ID | 18834224 |
Related Faculty:
Momin Siddiqui, M.D.