|Title||Productive infection of cultured human lymphoid cells by adenovirus.|
|Publication Type||Journal Article|
|Year of Publication||1987|
|Authors||Lavery D, Fu SM, Lufkin T, Chen-Kiang S|
|Date Published||1987 May|
|Keywords||Adenoviruses, Human, Cells, Cultured, DNA Replication, DNA, Viral, Genetic Vectors, Humans, Lymphocytes, RNA, Viral, Viral Plaque Assay, Viral Proteins, Virus Replication|
We investigated infection of cultures from established human B- and T-cell lines by adenoviruses. Infection by adenovirus type 2 or 5 was productive by the criteria of viral DNA replication, RNA synthesis, immunofluorescent staining of viral proteins, and assembly of biologically active virions. Whereas the kinetics of infection were reproducible and characteristic for each cell line, there appeared to be no correlation between the kinetics of infection and the origin from which the cell lines were established. In a myeloma and a T-cell line, the kinetics of infection approached those in HeLa cells. The presence of the Epstein-Barr virus genome in B lymphoid cells was not a prerequisite for adenoviral infection. Furthermore, expression of the E1A gene was repressed in myeloma cells in comparison with HeLa cells.
|Alternate Journal||J Virol|
|PubMed Central ID||PMC254124|
|Grant List||AI 19311 / AI / NIAID NIH HHS / United States |
AI 24404 / AI / NIAID NIH HHS / United States
CA 34546 / CA / NCI NIH HHS / United States
Selina Chen-Kiang, Ph.D.