Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists.

TitlePhospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists.
Publication TypeJournal Article
Year of Publication1989
AuthorsDiaz-Meco MT, Larrodera P, Lopez-Barahona M, Cornet ME, Barreno PG, Moscat J
JournalBiochem J
Date Published1989 Oct 01
KeywordsAluminum, Aluminum Chloride, Aluminum Compounds, Animals, Atropine, Carbachol, Cells, Cultured, Chlorides, Cyclic AMP, Fibroblasts, Hydrolysis, Mice, Parasympathomimetics, Phosphatidylcholines, Receptors, Muscarinic, Sodium Fluoride, Type C Phospholipases

The phospholipase C-catalysed breakdown of inositol-containing phospholipids is an important source of diacylglycerol in cells stimulated by several agonists. However, recent experimental evidence suggests that major phospholipids such as phosphatidylcholine may also be substrates of the phosphodiesteratic hydrolysis activated by hormones, growth factors and oncogene products. We show here that stimulation of muscarinic agonists activates the release of phosphocholine, which, along with diacylglycerol, is a metabolic product of phospholipase C-mediated hydrolysis of phosphatidylcholine. Fluoroaluminates mimic this muscarinic effect, strongly suggesting that carbachol-activated release of phosphocholine may be mediated by a guanine-nucleotide-binding protein. Evidence for this was obtained from experiments using permeabilized cells in which non-hydrolysable analogues of GTP activated phosphocholine release synergistically with carbachol.

Alternate JournalBiochem J
PubMed ID2557819
PubMed Central IDPMC1133397
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Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.

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